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simple ch ip kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc simple ch ip kit
    Simple Ch Ip Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simple+ch+ip+kit/pmc12908322-165-6-9?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    simple ch ip kit - by Bioz Stars, 2026-08
    86/100 stars

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    Srebp1 is critical for mTOR signaling to activate Me1 transcription, maintain NADPH homeostasis, and suppress ferroptosis in liver. A) Immunoblots of hepatic Srebp1, S6K1, p‐S6K1, and Me1 were measured in I/R‐treated mice with bpV or 3BDO injection. B) Enrichment of SREBP1 binding to the ME1 promoter was measured <t>by</t> <t>ChIP‐qPCR</t> analysis in HEK293 cells. C) Luciferase reporter assay in HEK293 cells transfected with a ME1‐luciferase construct in the presence of SREBP1 or empty vector alone. D) Immunoblots of hepatic Srebp1 and Me1 in mice treated with AAV8‐Srebp1 or AAV8‐GFP. E,F) Ratios of NADPH/NADP + (E) and GSH/GSSG (F) were measured in the livers of I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. G) Hepatic cysteine contents were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. H,I) Hepatic Ptgs2 mRNA (H) and MDA levels (I) were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. n = 6 per group. J–L) Serum levels of ALT (J), AST (K), and LDH (L) were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. n = 6 per group. M) Representative H&E‐stained liver sections from indicated mice. N–P) Serum levels of ALT (N), AST (O), and LDH (P) were measured in I/R‐treated Me1 Alb/Alb mice with or without AAV‐mediated Srebp1 overexpression. n = 5, 6. Q) Representative H&E‐stained liver sections from indicated mice. Significance was calculated by Student's t ‐test; * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Srebp1 is critical for mTOR signaling to activate Me1 transcription, maintain NADPH homeostasis, and suppress ferroptosis in liver. A) Immunoblots of hepatic Srebp1, S6K1, p‐S6K1, and Me1 were measured in I/R‐treated mice with bpV or 3BDO injection. B) Enrichment of SREBP1 binding to the ME1 promoter was measured <t>by</t> <t>ChIP‐qPCR</t> analysis in HEK293 cells. C) Luciferase reporter assay in HEK293 cells transfected with a ME1‐luciferase construct in the presence of SREBP1 or empty vector alone. D) Immunoblots of hepatic Srebp1 and Me1 in mice treated with AAV8‐Srebp1 or AAV8‐GFP. E,F) Ratios of NADPH/NADP + (E) and GSH/GSSG (F) were measured in the livers of I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. G) Hepatic cysteine contents were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. H,I) Hepatic Ptgs2 mRNA (H) and MDA levels (I) were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. n = 6 per group. J–L) Serum levels of ALT (J), AST (K), and LDH (L) were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. n = 6 per group. M) Representative H&E‐stained liver sections from indicated mice. N–P) Serum levels of ALT (N), AST (O), and LDH (P) were measured in I/R‐treated Me1 Alb/Alb mice with or without AAV‐mediated Srebp1 overexpression. n = 5, 6. Q) Representative H&E‐stained liver sections from indicated mice. Significance was calculated by Student's t ‐test; * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Srebp1 is critical for mTOR signaling to activate Me1 transcription, maintain NADPH homeostasis, and suppress ferroptosis in liver. A) Immunoblots of hepatic Srebp1, S6K1, p‐S6K1, and Me1 were measured in I/R‐treated mice with bpV or 3BDO injection. B) Enrichment of SREBP1 binding to the ME1 promoter was measured by ChIP‐qPCR analysis in HEK293 cells. C) Luciferase reporter assay in HEK293 cells transfected with a ME1‐luciferase construct in the presence of SREBP1 or empty vector alone. D) Immunoblots of hepatic Srebp1 and Me1 in mice treated with AAV8‐Srebp1 or AAV8‐GFP. E,F) Ratios of NADPH/NADP + (E) and GSH/GSSG (F) were measured in the livers of I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. G) Hepatic cysteine contents were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. H,I) Hepatic Ptgs2 mRNA (H) and MDA levels (I) were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. n = 6 per group. J–L) Serum levels of ALT (J), AST (K), and LDH (L) were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. n = 6 per group. M) Representative H&E‐stained liver sections from indicated mice. N–P) Serum levels of ALT (N), AST (O), and LDH (P) were measured in I/R‐treated Me1 Alb/Alb mice with or without AAV‐mediated Srebp1 overexpression. n = 5, 6. Q) Representative H&E‐stained liver sections from indicated mice. Significance was calculated by Student's t ‐test; * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Advanced Science

    Article Title: Malic Enzyme 1 as a Novel Anti‐Ferroptotic Regulator in Hepatic Ischemia/Reperfusion Injury

    doi: 10.1002/advs.202205436

    Figure Lengend Snippet: Srebp1 is critical for mTOR signaling to activate Me1 transcription, maintain NADPH homeostasis, and suppress ferroptosis in liver. A) Immunoblots of hepatic Srebp1, S6K1, p‐S6K1, and Me1 were measured in I/R‐treated mice with bpV or 3BDO injection. B) Enrichment of SREBP1 binding to the ME1 promoter was measured by ChIP‐qPCR analysis in HEK293 cells. C) Luciferase reporter assay in HEK293 cells transfected with a ME1‐luciferase construct in the presence of SREBP1 or empty vector alone. D) Immunoblots of hepatic Srebp1 and Me1 in mice treated with AAV8‐Srebp1 or AAV8‐GFP. E,F) Ratios of NADPH/NADP + (E) and GSH/GSSG (F) were measured in the livers of I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. G) Hepatic cysteine contents were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. H,I) Hepatic Ptgs2 mRNA (H) and MDA levels (I) were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. n = 6 per group. J–L) Serum levels of ALT (J), AST (K), and LDH (L) were measured in I/R‐treated mice with or without AAV‐mediated Srebp1 overexpression. n = 6 per group. M) Representative H&E‐stained liver sections from indicated mice. N–P) Serum levels of ALT (N), AST (O), and LDH (P) were measured in I/R‐treated Me1 Alb/Alb mice with or without AAV‐mediated Srebp1 overexpression. n = 5, 6. Q) Representative H&E‐stained liver sections from indicated mice. Significance was calculated by Student's t ‐test; * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: ChIP was performed using the Simple Ch‐IP Plus Enzymatic Chromatin IP Kit (Cell Signaling) in accordance with the manufacturer's instructions.

    Techniques: Western Blot, Injection, Binding Assay, ChIP-qPCR, Luciferase, Reporter Assay, Transfection, Construct, Plasmid Preparation, Over Expression, Staining